PCR——实验室必备实验，从基因鉴定到信号通路验证，几乎每周都要做那么几次。 然而，各种PCR技术，包括PCR、qPCR、RT-PCR、RT-qPCR、Real-Time PCR和dPCR，长得实在是太像了，好多同学看得头晕眼花也没弄明白这几种PCR有什么区别。 今天，一篇文章，带你清清楚楚 ...

Reverse transcription quantitative real-time PCR (RT-qPCR) is the most commonly used method for the quantification of mRNA expression levels. Generally, the starting material for RTqPCR is purified ...

精准识别表皮生长因子受体（EGFR）基因突变对于非小细胞肺癌（NSCLC）患者的个体化治疗至关重要。当下市场上，存在多种用于检测这些基因突变的方法和仪器，然而其检测性能参差不齐，尤其是在检测少见 EGFR 基因突变方面，差异更为明显。近日，Lung Cancer ...

为解决LNP-mRNA药物缺乏针对性药代动力学（PK）分析指南的问题，研究人员系统开展了RT-qPCR技术验证研究，建立了涵盖引物设计、样本处理、标准曲线构建的全流程标准化方案，证实该方法可精准定量循环中mRNA浓度，为RNA疗法临床转化提供了关键技术支持。

CINCINNATI, Dec. 03, 2020 (GLOBE NEWSWIRE) -- Meridian Bioscience, Inc. (NASDAQ: VIVO), a leading global provider of diagnostic testing solutions and life science raw materials, announced today that ...

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

(MENAFN- GlobeNewsWire - Nasdaq) CINCINNATI, Dec. 03, 2020 (GLOBE NEWSWIRE) -- Meridian Bioscience, Inc. (NASDAQ: VIVO), a leading global provider of diagnostic testing solutions and life science raw ...

In a recent study published in PLOS ONE, researchers developed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system to identify severe acute respiratory syndrome coronavirus ...

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